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Fluorescence Klebsiella Oxytoca Atcc43086, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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saha  (MedChemExpress)
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XQ2 has synergistic effects with several LRAs on the reactivation of latent HIV-1. J-Lat A2 (A, B) and ACH2 (C, D) cells were stimulated with PMA (10 ng/mL), XQ2 (30 μM), prostratin (0.5 μM), <t>SAHA</t> (0.5 <t>μM),</t> <t>JQ1</t> (0.5 μM), or combinations therein for 48 h. The percentage of GFP-positive cells or p24 antigen was measured by flow cytometry or ELISA, and the corresponding cell viability was examined by MTT assay. The synergy of the LRA combinations was calculated by using the Bliss independence model. Δf axy = 0, Δf axy > 0, and Δf axy < 0 indicate additive, synergistic, and antagonistic effects, respectively. The data represent the means ± SDs of three independent experiments. One-way ANOVA followed by Dunnett’s multiple comparison post hoc test was used to statistically analyze the differences between the XQ2 or prostratin, SAHA, JQ1 alone, and combination groups (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).
Saha, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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XQ2 has synergistic effects with several LRAs on the reactivation of latent HIV-1. J-Lat A2 (A, B) and ACH2 (C, D) cells were stimulated with PMA (10 ng/mL), XQ2 (30 μM), prostratin (0.5 μM), <t>SAHA</t> (0.5 <t>μM),</t> <t>JQ1</t> (0.5 μM), or combinations therein for 48 h. The percentage of GFP-positive cells or p24 antigen was measured by flow cytometry or ELISA, and the corresponding cell viability was examined by MTT assay. The synergy of the LRA combinations was calculated by using the Bliss independence model. Δf axy = 0, Δf axy > 0, and Δf axy < 0 indicate additive, synergistic, and antagonistic effects, respectively. The data represent the means ± SDs of three independent experiments. One-way ANOVA followed by Dunnett’s multiple comparison post hoc test was used to statistically analyze the differences between the XQ2 or prostratin, SAHA, JQ1 alone, and combination groups (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).
Hdac Inhibitor Saha, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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XQ2 has synergistic effects with several LRAs on the reactivation of latent HIV-1. J-Lat A2 (A, B) and ACH2 (C, D) cells were stimulated with PMA (10 ng/mL), XQ2 (30 μM), prostratin (0.5 μM), <t>SAHA</t> (0.5 <t>μM),</t> <t>JQ1</t> (0.5 μM), or combinations therein for 48 h. The percentage of GFP-positive cells or p24 antigen was measured by flow cytometry or ELISA, and the corresponding cell viability was examined by MTT assay. The synergy of the LRA combinations was calculated by using the Bliss independence model. Δf axy = 0, Δf axy > 0, and Δf axy < 0 indicate additive, synergistic, and antagonistic effects, respectively. The data represent the means ± SDs of three independent experiments. One-way ANOVA followed by Dunnett’s multiple comparison post hoc test was used to statistically analyze the differences between the XQ2 or prostratin, SAHA, JQ1 alone, and combination groups (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).
Vorinostat, supplied by TargetMol, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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species isolate id strain info species isolate id strain info klebsiella oxytoca atcc 8724 n  (ATCC)
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XQ2 has synergistic effects with several LRAs on the reactivation of latent HIV-1. J-Lat A2 (A, B) and ACH2 (C, D) cells were stimulated with PMA (10 ng/mL), XQ2 (30 μM), prostratin (0.5 μM), <t>SAHA</t> (0.5 <t>μM),</t> <t>JQ1</t> (0.5 μM), or combinations therein for 48 h. The percentage of GFP-positive cells or p24 antigen was measured by flow cytometry or ELISA, and the corresponding cell viability was examined by MTT assay. The synergy of the LRA combinations was calculated by using the Bliss independence model. Δf axy = 0, Δf axy > 0, and Δf axy < 0 indicate additive, synergistic, and antagonistic effects, respectively. The data represent the means ± SDs of three independent experiments. One-way ANOVA followed by Dunnett’s multiple comparison post hoc test was used to statistically analyze the differences between the XQ2 or prostratin, SAHA, JQ1 alone, and combination groups (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).
Species Isolate Id Strain Info Species Isolate Id Strain Info Klebsiella Oxytoca Atcc 8724 N, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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klebsiella oxytoca atcc 43086 n  (ATCC)
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XQ2 has synergistic effects with several LRAs on the reactivation of latent HIV-1. J-Lat A2 (A, B) and ACH2 (C, D) cells were stimulated with PMA (10 ng/mL), XQ2 (30 μM), prostratin (0.5 μM), <t>SAHA</t> (0.5 <t>μM),</t> <t>JQ1</t> (0.5 μM), or combinations therein for 48 h. The percentage of GFP-positive cells or p24 antigen was measured by flow cytometry or ELISA, and the corresponding cell viability was examined by MTT assay. The synergy of the LRA combinations was calculated by using the Bliss independence model. Δf axy = 0, Δf axy > 0, and Δf axy < 0 indicate additive, synergistic, and antagonistic effects, respectively. The data represent the means ± SDs of three independent experiments. One-way ANOVA followed by Dunnett’s multiple comparison post hoc test was used to statistically analyze the differences between the XQ2 or prostratin, SAHA, JQ1 alone, and combination groups (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).
Klebsiella Oxytoca Atcc 43086 N, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fluorescence enterobacter aerogenes atcc 13048  (ATCC)
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XQ2 has synergistic effects with several LRAs on the reactivation of latent HIV-1. J-Lat A2 (A, B) and ACH2 (C, D) cells were stimulated with PMA (10 ng/mL), XQ2 (30 μM), prostratin (0.5 μM), <t>SAHA</t> (0.5 <t>μM),</t> <t>JQ1</t> (0.5 μM), or combinations therein for 48 h. The percentage of GFP-positive cells or p24 antigen was measured by flow cytometry or ELISA, and the corresponding cell viability was examined by MTT assay. The synergy of the LRA combinations was calculated by using the Bliss independence model. Δf axy = 0, Δf axy > 0, and Δf axy < 0 indicate additive, synergistic, and antagonistic effects, respectively. The data represent the means ± SDs of three independent experiments. One-way ANOVA followed by Dunnett’s multiple comparison post hoc test was used to statistically analyze the differences between the XQ2 or prostratin, SAHA, JQ1 alone, and combination groups (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).
Fluorescence Enterobacter Aerogenes Atcc 13048, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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klebsiella oxytoca atcc  (ATCC)
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XQ2 has synergistic effects with several LRAs on the reactivation of latent HIV-1. J-Lat A2 (A, B) and ACH2 (C, D) cells were stimulated with PMA (10 ng/mL), XQ2 (30 μM), prostratin (0.5 μM), <t>SAHA</t> (0.5 <t>μM),</t> <t>JQ1</t> (0.5 μM), or combinations therein for 48 h. The percentage of GFP-positive cells or p24 antigen was measured by flow cytometry or ELISA, and the corresponding cell viability was examined by MTT assay. The synergy of the LRA combinations was calculated by using the Bliss independence model. Δf axy = 0, Δf axy > 0, and Δf axy < 0 indicate additive, synergistic, and antagonistic effects, respectively. The data represent the means ± SDs of three independent experiments. One-way ANOVA followed by Dunnett’s multiple comparison post hoc test was used to statistically analyze the differences between the XQ2 or prostratin, SAHA, JQ1 alone, and combination groups (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).
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XQ2 has synergistic effects with several LRAs on the reactivation of latent HIV-1. J-Lat A2 (A, B) and ACH2 (C, D) cells were stimulated with PMA (10 ng/mL), XQ2 (30 μM), prostratin (0.5 μM), SAHA (0.5 μM), JQ1 (0.5 μM), or combinations therein for 48 h. The percentage of GFP-positive cells or p24 antigen was measured by flow cytometry or ELISA, and the corresponding cell viability was examined by MTT assay. The synergy of the LRA combinations was calculated by using the Bliss independence model. Δf axy = 0, Δf axy > 0, and Δf axy < 0 indicate additive, synergistic, and antagonistic effects, respectively. The data represent the means ± SDs of three independent experiments. One-way ANOVA followed by Dunnett’s multiple comparison post hoc test was used to statistically analyze the differences between the XQ2 or prostratin, SAHA, JQ1 alone, and combination groups (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).

Journal: ACS Omega

Article Title: XQ2, a Novel Derivative of Resveratrol, Reactivates Latent HIV‑1 via the Activation of Positive Transcription Elongation Factor B

doi: 10.1021/acsomega.5c10420

Figure Lengend Snippet: XQ2 has synergistic effects with several LRAs on the reactivation of latent HIV-1. J-Lat A2 (A, B) and ACH2 (C, D) cells were stimulated with PMA (10 ng/mL), XQ2 (30 μM), prostratin (0.5 μM), SAHA (0.5 μM), JQ1 (0.5 μM), or combinations therein for 48 h. The percentage of GFP-positive cells or p24 antigen was measured by flow cytometry or ELISA, and the corresponding cell viability was examined by MTT assay. The synergy of the LRA combinations was calculated by using the Bliss independence model. Δf axy = 0, Δf axy > 0, and Δf axy < 0 indicate additive, synergistic, and antagonistic effects, respectively. The data represent the means ± SDs of three independent experiments. One-way ANOVA followed by Dunnett’s multiple comparison post hoc test was used to statistically analyze the differences between the XQ2 or prostratin, SAHA, JQ1 alone, and combination groups (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).

Article Snippet: Small-molecule inhibitors such as Resveratrol, JQ1, and SAHA were sourced from MedChemExpress (MCE, Vantaa, Finland).

Techniques: Flow Cytometry, Enzyme-linked Immunosorbent Assay, MTT Assay, Comparison